Oxidant‐mediated proteolytic activation of Ca2+‐ATPase in microsomes of pulmonary smooth muscle

FEBS Letters

Ghosh, Salil K.²  Michael, John R.¹  Chakraborti, Sajal²  Chakraborti, Tapati² 

[1] Department of Medicine, University of Utah, Salt Lake City, UT 84132, USA;Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, India

关键词: Ca2+-ATPase;    Protease;    Aprotinin;    Pulmonary smooth muscle tissue;    Microsome;    Oxidant;    Hydrogen peroxide;    HBPS;    Hank's buffered physiological saline;    BAPNA;   

DOI  :  10.1016/0014-5793(96)00471-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of bovine pulmonary artery smooth muscle tissue microsomes with H₂O₂ (1 mM) markedly stimulated protease activity tested with a synthetic substrate p-nitroanilide (BAPNA), and also enhanced Ca²⁺-ATPase activity. ATP-dependent Ca²⁺ uptake was found to be stimulated upon treatment of the microsomes with H₂O₂. Pretreatment of the microsomes with vitamin E and aprotinin prevented the H₂O₂-induced stimulation of Ca²⁺-ATPase activity and also ATP-dependent Ca²⁺ uptake. In contrast, H₂O₂-induced inhibition of Na⁺-dependent Ca²⁺ uptake was reversed by vitamin E and aprotinin.

【 授权许可】

Unknown