Prevention of growth arrest‐induced cell death of vascular smooth muscle cells by a product of growth arrest‐specific gene, gas6

FEBS Letters

Kawamoto, Keiko¹  Nakano, Toru¹  Higashino, Ken-ichi¹  Arita, Hitoshi¹ 

[1] Discovery Research Laboratories II, Shionogi < Co., Ltd., 5-12-4 Sagisu, Fukushima-ku, Osaka 553, Japan

关键词: Vascular smooth muscle cell;    Cell death;    Gas6;    Vitamin K;    Growth factor;    Survival factor;    VSMC;    vascular smooth muscle cell;    Gla;    γ-carboxyglutamic acid;    PDGF;    platelet-derived growth factor;    bFGF;    basic fibroblast growth factor;    EGF;    epidermal growth factor;    HB-EGF;    heparin-binding EGF-like growth factor;    DMEM;    Dulbecco's modified Eagle's medium;    BSA;    bovine serum albumin;    MTT;    3-(4;    5dimethylthiazol-2-yl)-2;    5-diphenyltetrazolium bromide;    LDH;    lactate dehydrogenase;   

DOI  :  10.1016/0014-5793(96)00395-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have purified Gas6 as a growth-potentiating factor for vascular smooth muscle cells (VSMCs) [Nakano, T. et al. (1995) J. Biol. Chem. 270, 5702-57051. However, specific production of Gas6 in growth-arrested cells raises an intriguing question as to the physiological function of Gas6. In this study, we found that serum-starved VSMCs secreted some survival factors and depletion of the factors induced cell death of VSMCs. Finally, we demonstrated that cell death was prevented by the addition of Gas6, suggesting that one of the major biological activity of Gas6 is protection of growth-arrested VSMCs from death.

【 授权许可】

Unknown