Translational fusion of heat labile enterotoxin chain B and β‐subunit of human chorionic gonadotropin: periplasmic expression in Escherichia coli and its immunogenicity

FEBS Letters

Pillai, Devika¹  Alok, Deoraj²  Dixit, Aparna¹  Garg, Lalit C.² 

[1] Department of Zoology, Delhi University, Delhi-110 007, India;National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi-110 067, India

关键词: Periplasmic expression;    Fusion protein;    βhCG;    Signal sequence;    Immunogenicity;   

DOI  :  10.1016/0014-5793(96)00428-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A fusion gene was constructed consisting of heat labile enterotoxin chain B (LTB) of E. coli genetically linked at its C-terminus to the β-subunit of human chorionic gonadotropin in translational fusion, under the control of tac promoter and LTB signal sequence. Expression of the fusion gene (about 5 μg/ml) in E. coli was confirmed by immunoblot analysis using both anti-LTB and anti-βhCG polyclonal antibodies. The fusion protein was efficiently processed and exported to the periplasmic space. LTB in the fusion protein retained its ability to bind to GM1 ganglioside receptor. Mice immunized with the fusion protein produce antibodies that recognize recombinant βhCG and the native hCG suggesting its potential use as a contraceptive vaccine.

【 授权许可】

Unknown