Very low density lipoprotein receptor binds apolipoprotein E2/2 as well as apolipoprotein E3/3

FEBS Letters

Ookubo, Minoru¹  Nakai, Tsuguhiko³  Yamamoto, Tokuo²  Takahashi, Sadao³  Oida, Koji³  Kohno, Mitsuyuki³  Suzuki, Jinya³  Murase, Toshio¹ 

[1]Department of Endocrinology and Metabolism, Toranomon Hospital, Tokyo 113, Japan

[2]Tohoku University Gene Research Center, Sendai 981, Japan

[3]Third Department of Internal Medicine, Fukui Medical School, Fukui 910-11, Japan

关键词: Lipoprotein;    Lipoprotein receptor;    Very low density lipoprotein;    VLDL receptor;    Apolipoprotein E;    VLDL;    very low density lipoprotein;    LDL;    low density lipoprotein;    apo;    apolipoprotein;    β-VLDL;    β-migrating very low density lipoprotein;    LPL;    lipoprotein lipase;    PCR;    polymerase chain reaction;    LPDS;    lipoprotein-deficient serum;    LRP;    LDL receptor-related protein;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/0014-5793(96)00439-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins. The human VLDL receptor was overexpressed in ldlA-7, a mutant Chinese hamster ovary cells lacking LDL receptors. Each VLDL obtained from a normolipidemic subject with two ε3 or ε2 alleles similarly competed for the binding of radiolabeled rabbit β-VLDL to the VLDL receptors. The anti-apoE monoclonal antibody 1D7, which inhibited binding of apoE3 to the LDL receptors, failed to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL receptors. Results indicate that the binding site of apoE on the VLDL receptor may differ from its binding site on the LDL receptor.

【 授权许可】

Unknown