The vacuolar ATPase proton pump is required for the cytotoxicity of Bacillus anthracis lethal toxin

FEBS Letters

Montecucco, Cesare³  Ménard, Armelle³  Altendorf, Karlheinz²  Breves, Daniel³  Mock, Michèle¹ 

[1] Laboratoire de Génétique Moléculaire des Toxines (URA 1858, CNRS), Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, France;Universität Osnabrück, Fachbereich Biologie/Chemie, Postfach 4469, D-4500 Osnabrück, Germany;Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, Via Trieste 75, 35121 Padua, Italy

关键词: Lethal factor;    Anthrax;    Toxin;    Translocation;    V-ATPase;    Endosome;    PA;    protective antigen;    LF;    lethal factor;    EF;    edema factor;    PBS;    phosphate buffered saline;    DMF;    dimethylformamide;    SDS;    sodium dodecyl sulfate;    BafA1;    bafilomycin A1;    Conc A;    concanamycin A;    V-ATPase;    vacuolar ATPase proton pump;   

DOI  :  10.1016/0014-5793(96)00422-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nature of the cytopathic effect exerted by the lethal factor toxin (LF) of Bacillus anthracis on sensitive cells is unknown. The toxin requires the passage through acidic vesicles in order to exert its effect within the cytosol. Here, we show that bafilomycins and concanamycin A, selective inhibitors of the vacuolar ATPase proton pump, are the most powerful known inhibitors of LF macrophage toxicity. These inhibitors are fully active long after LF addition to macrophages, suggesting that LF enters the cytosol after having reached a late endosomal compartment.

【 授权许可】

Unknown