Molecular characterization of a new urea transporter in the human kidney

FEBS Letters

Ripoche, Pierre¹  Rousselet, Germain¹  Olivès, Bernadette³  Bailly, Pascal³  Cartron, Jean-Pierre³  Mattei, Marie-Geneviève²  Matassi, Giorgio³  Martial, Sonia¹ 

[1] Service de Biologie Cellulaire, C.E.A./Saclay, 91191 Gif-sur-Yvette, France;INSERM U242, Hôpital de la Timone, 13385 Marseille, France;INSERM U76, GIP-Institut National de la Transfusion Sanguine, 6 rue Alexandre Cabanel, 75015 Paris, France

关键词: Urea transport;    Human kidney;    Tissue expression;    Transcription-translation assay;    In situ hybridization;   

DOI  :  10.1016/0014-5793(96)00425-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone (HUT2) sharing 61.1% and 89.9% sequence identity with the human erythroid (HUT11) and the rabbit (UT2) urea transporters, respectively, was isolated by homology cloning from a human kidney library. HUT2 transcripts were restricted to the kidney and the HUT2 polypeptide was not immunoprecipitated with blood group Kidd-related antibodies (anti-Jk3) in coupled transcription-translation assays. Functional expression studies in Xenopus oocytes demonstrated that HUT2-mediated urea transport was not inhibited by p-chloromercuribenzene sulfonate (pCMBS) which, however, inhibited the urea flux mediated by HUT11. These findings demonstrate that at least two distinct urea transporters are present in human tissues. By in situ hybridization, the gene encoding HUT2 has been assigned to chromosome 18q12.1-q21-1, as found previously for the Kidd/urea transporter HUT11, suggesting that both genes evolved from duplication of a common ancestor.

【 授权许可】

Unknown