Expression of GCAP 1 and GCAP2 in the retinal degeneration (rd) mutant chicken retina

FEBS Letters

Helekar, Bharati S.³  Baehr, Wolfgang¹  Palczewski, Krzysztof²  Ruiz, Claudia C.³  Semple-Rowland, Susan L.⁴  Gorczyca, Wojciech A.²  Buczylko, Janina²  Subbaraya, Iswari³ 

[1] Moran Eye Center, University of Utah Health Science Center, Salt Lake City, UT 84132, USA;Departments of Ophthalmology and Pharmacology, University of Washington, Seattle, WA 98195, USA;Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA;Department of Neuroscience, University of Florida College of Medicine, Gainesville, FL 32610, USA

关键词: Guanylate cyclase activating protein;    rd chicken;    Inherited retinal disease;    Calcium;    Photoreceptor;    cGMP;   

DOI  :  10.1016/0014-5793(96)00345-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned the guanylate cyclase activating proteins, GCAP1 and GCAP2, from chicken retina and examined their expression in normal and predegenerate rd/rd chicken retina. Northern analyses show that the amounts of the single transcripts encoding GCAPI and GCAP2 are reduced to about 70% of normal levels in rdlrd retina. Western analyses reveal that GCAP2 levels appear normal in this retina, while GCAPI levels are reduced by more than 90%. The specific downregulation of GCAPI in rd/rd retina is consistent with a model for this disease in which activation of guanylate cyclase in the photoreceptors is abnormal, resulting in low levels of cGMP and an absence of phototransduction.

【 授权许可】

Unknown