FEBS Letters | |
Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus | |
Erdin, Christian1  Sprenger, Norbert1  Lüscher, Marcel1  Hochstrasser, Urs1  Wiemken, Andres1  Boller, Thomas1  | |
[1] Department of Botany, University of Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland | |
关键词: Fructan; Fructan-fructan 1-fructosyltransferase; Helianthus tuberosus; Inulin; Sucrose-sucrose 1-fructosyltransferase; 1-FFT; fructan-fructan 1-fructosyltransferase; 6-SFT; sucrose-fructan 6-fructosyltransferase; 1-SST; sucrose-sucrose 1-fructosyltransferase; IEF; isoelectric focusing; | |
DOI : 10.1016/0014-5793(96)00343-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Sucrose-sucrose 1-fructosyltransferase (1-SST) was purified 100-fold from tubers of Helianthus tuberosus L. The purified enzyme was essentially devoid of invertase activity and could be separated by isoelectric focusing into five isoforms which all were composed of two subunits (59 and 26 kDa). Fructan-fructan 1-fructosyltransferase (1-FFT) was purified from the same source [M. Lüscher et al. (1993) New Phytologist 123, 437–442). When incubated individually with sucrose, 1-FFT was inactive while 1-SST formed isokestose (trimer) and, upon prolonged incubation, some nystose (tetramer). When a combination of the two enzymes was incubated with sucrose, a series of oligofructosides with a degree of polymerization of up to 20 was formed. Amino acid sequences of tryptic peptide fragments from both 1-SST and 1-FFT indicate that these enzymes are highly homologous with plant invertases.
【 授权许可】
Unknown
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