The structural origin of the color differences in the bioluminescence of firefly luciferase

FEBS Letters

关键词: Luciferase;    Firefly;    Light emitting;    Color difference;    Active site;    IPTG;    isopropyl β-d-thiogalactopyranoside;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(96)00288-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Six chimeric mutants between Hotaria parvula (λmax=568 nm) and Pyrocoelia miyako (λmax=550 nm) luciferases were reconstructed to determine the structural origin of the color differences in firefly luciferase. Based on light-emitting color, five chimeric luciferases could be divided into two groups: the three green-emitting mutants, classified as P. miyako luciferase, and the two yellow-emitting mutants, classified as H. parvula luciferase. Their common fragments between Val-209 and Ala-318 within each group contain the active site for the color differences.

【 授权许可】

Unknown