Protective effect of lysozyme‐galactomannan or lysozyme‐palmitic acid conjugates against Edwardsiella tarda infection in carp, Cyprinus carpio L

FEBS Letters

Kato, Akio³  Gohya, Yasuhide²  Losso, Jack N.¹  Nakai, Shuryo¹  Nakamura, Soichiro¹ 

[1] Department of Food Science, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada;Laboratory of Environmental Science, Ube College, Yamaguchi 755, Japan;Department of Biochemistry, Yamaguchi University, Yamaguchi 753, Japan

关键词: Lysozyme-galactomannan conjugate;    Lysozyme-palmitic acid conjugate;    Bacterial infection;    Fish therapeutic;   

DOI  :  10.1016/0014-5793(96)00260-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The protective effect of lysozyme-galactomannan or lysozyme-palmitic acid conjugates orally administered to carp, Cyprinus carpio L. was investigated using a virulent strain of Gram-negative Edwardsiella tarda isolated from an infected fish. Lysozyme-galactomannan conjugate was prepared through controlled Maillard reaction. Lysozyme-palmitic acid conjugate was prepared through base-catalyzed ester exchange using N-hydroxysuccinimide ester of palmitic acid. The conjugates provided substantial protection to carp infected with a Gram-negative bacteria fish pathogen E. tarda NG 8104. Lytic activities of lysozyme conjugates with galactomannan and palmitic acid were about 80 and 71% of native lysozyme using Micrococcus lysodeikticus as a substrate. Feeding with lysozyme conjugates, for 8 days, significantly enhanced fish protection against E. tarda infection. The survival rate was 30% for lysozyme-galactomannan conjugate treated fish and 20% for lysozyme-palmitic acid conjugate treated fish after 6 days cultivation while all control fish died within 3 days. On the other hand, a recovery rate of 40% after 6 days was observed in the fish group that were fed lysozyme-palmitic acid conjugate 3 and 2 h before and after E. tarda challenge, respectively, and for 6 consecutive days. The results of this work show the possibility of utilizing lysozyme conjugates with galactomannan or palmitic acid as a therapeutic for infection in fish.

【 授权许可】

Unknown