Characterization of the dystrophin‐syntrophin interaction using the two‐hybrid system in yeast

FEBS Letters

Castelló, Anna¹  Gilgenkrantz, Hélène¹  Brochériou, Valérie¹  Chafey, Philippe¹  Kahn, Axel¹ 

[1] U. 129 INSERM, Institut Cochin de Génétique Moléculaire, 24 rue du Fbg St. Jacques, 75014 Paris, France

关键词: Protein-protein interaction;    59 DAP;    Duchenne muscular dystrophy;   

DOI  :  10.1016/0014-5793(96)00214-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The carboxy-terminal region of dystrophin has previously been shown to interact directly with α1 syntrophin, a cytoplasmic component of the dystrophin-glycoprotein complex, by in vitro biochemical studies such as overlay assay or immunoprecipitation. Using the two-hybrid system, we have isolated from a human heart cDNA library the entire coding sequence of human α1 syntrophin, therefore confirming for the first time this interaction via an in vivo approach. In addition, we have reduced the interaction domain to the distal half of α1 syntrophin.

【 授权许可】

Unknown