Differential regulation of cardiac heme oxygenase‐1 and vascular endothelial growth factor mRNA expressions by hemin, heavy metals, heat shock and anoxia

FEBS Letters

Eyssen-Hernandez, Rodrigo¹  Frelin, Christian¹  Ladoux, Annie¹ 

[1]Institut de Pharmacologie Moléculaire et Cellulaire du CNRS, Université de Nice-Sophia Antipolis, 660 Route des Lucioles, 06560 Valbonne, France

关键词: Hypoxia;    Vascular endothelial growth factor;    Heme oxygenase;    Cobalt;    Cadmium;    Heat shock;   

DOI  :  10.1016/0014-5793(96)00127-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Increasing attention has been paid to the effects of hypoxia, heavy metals and heat shocks on gene expression and to the similarities in their actions. This paper compares mRNA levels of two putative hypoxia, heavy metal and heat shock sensitive genes: heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) in myocyte-enriched cultures of neonatal rat heart cells. HO-1 mRNA expression is stimulated by hemin, Cd²⁺, Co²⁺ and heat shocks but not by Ni²⁺ or Mn²⁺. It is stimulated by long (13 h) but not by short (4 h) periods of anoxia. Conversely, VEGF mRNA expression is stimulated by short as well as long periods of anoxia, by Cd²⁺, Co²⁺, Ni²⁺ and Mn²⁺ but not by hemin or heat shocks. The results suggest that heavy metals, anoxia and heat shocks exert their effects on VEGF and HO-1 mRNA expression through separate though potentially overlapping mechanisms. Increased expressions of HO-1 and VEGF may be both cardioprotective under hypoxic/ischemic conditions.

【 授权许可】

Unknown