The role of glutathione in the transport and catabolism of nitric oxide

FEBS Letters

Singh, Ravinder J.¹  Kalyanaraman, B.¹  Hogg, Neil¹ 

[1] Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, WI 53226, USA

关键词: Nitric oxide;    Glutathione;    Nitrous oxide;    Peroxynitrite;    Nitrosothiol;   

DOI  :  10.1016/0014-5793(96)00086-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitric oxide acts as a neuronal and vascular messenger implying diffusion through intracellular environments containing 5–10 mM glutathione. Nitric oxide reacts with glutathione under aerobic conditions generating S-nitrosoglutathione (GSNO). GSNO reacts with glutathione (k = 8.3 × 10⁻³ M⁻¹ · s⁻¹) to generate nitrous oxide and glutathione disulfide (GSSG). Anaerobically, glutathione reacts with nitric oxide generating nitrous oxide and GSSG (k = 4.8 × 10⁻⁴ s⁻¹ at 5 mM GSH). In both aerobic and anaerobic situations the nitroxyl anion may be an intermediate in the synthesis of nitrous oxide and, under aerobic conditions, nitroxyl anion may generate peroxynitrite. We present a hypothesis for the intracellular interaction between nitric oxide and glutathione.

【 授权许可】

Unknown