Dictyostelium discoideum contains a family of calmodulin‐related EF‐hand proteins that are developmentally regulated

FEBS Letters

André, Bernadette¹  Schleicher, Michael¹  Noegel, Angelika A.² 

[1] Adolf-Butenandt-Institut/Zellbiologie, Schillerstr. 42, 80336 München, Germany;Max-Planck-Institut für Biochemie, 82152 Martinsried, Germany

关键词: Ca2+-binding protein;    Development;    Dictyostelium;    EF-hand protein;   

DOI  :  10.1016/0014-5793(96)00176-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length genomic DNA fragment that codes for a novel EF-hand protein from Dictyostelium discoideum was cloned and sequenced. The protein is composed of 168 amino acids and contains four consensus sequences that are typical for Ca²⁺-binding EF-hand domains. The protein sequence exhibits only minor similarities to other calmodulin-type proteins from Dictyostelium. The genomic DNA harbors two short introns; their positions suggest that the gene is unrelated to the EF-hand proteins from the calmodulin group. Northern blot analysis showed that the mRNA level was significantly increased during development. Polyclonal antibodies raised against the recombinant protein recognized in Western blots a protein of about 20 kDa. Like the mRNA, also the protein was more abundant in developing cells. Overlay experiments with ⁴⁵Ca²⁺ indicated that the EF-hands in fact have Ca²⁺-binding activity. The recent description of CBP1, another calmodulin-type Dictyostelium protein that is upregulated during development [Coukell et al. (1995) FEBS Lett. 362, 342–346], suggests that D. discoideum contains a family of EF-hand proteins that have specific functions during distinct steps of development. We therefore designate the protein described in this report as CBP2.

【 授权许可】

Unknown