Sphingosine‐1‐phosphate inhibits actin nucleation and pseudopodium formation to control cell motility of mouse melanoma cells

FEBS Letters

Yamamura, Soichiro²  Ruan, Fuqiang¹  Hakomori, Sen-itiroh¹  Sadahira, Yoshito²  Igarashi, Yasuyuki¹ 

[1] Departments of Pathobiology and Microbiology, University of Washington, Seattle, WA 98195, USA;The Biomembrane Institute, 201 Elliott Ave. W., Seattle, WA 98119, USA

关键词: Sphingosine-1-phosphate;    Actin nucleation;    Pseudopodium;    Cell motility;    C2-Cer;    C2-ceramide (N-acetyl sphingosine);    CM;    conditioned medium;    DMEM;    Dulbecco's modified Eagle's medium;    DMS;    N;    N-dimethylsphingosine;    DMS-1-P;    N;    N-dimethylsphingosine-1-phosphate;    EDTA;    ethylenediaminetetraacetic acid;    PBS;    phosphate-buffered saline;    SPC;    sphingosylphosphorylcholine;    Sph;    sphingosine;    Sph-1-P;    sphingosine-1-phosphate;    TMS;    N;    N;    N-trimethylsphingosine;    TMS-1-P;    N;    N-trimethylsphingosine-1-phosphate;    TPA;    12-O-tetradecanoylphorbol 13-acetate;   

DOI  :  10.1016/0014-5793(96)00175-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sphingosine-1-phosphate (Sph-1-P), the initial product of sphingosine (Sph) catabolism, has been reported to inhibit motility of mouse melanoma B16/F1 and other types of cells at very low concentrations (10–100 nM). Sph-1-P (100 nM–1 μM) inhibited pseudopodium formation by blocking polymerization and reorganization of actin filaments in newly formed pseudopodia, and reduced F-actin by ∼ 25% in F1 cells. A pyrene-labeled actin nucleation assay revealed that Sph-1-P (100 nM) inhibits actin nucleation mediated by F1 cell plasma membranes. These results suggest that Sph-1-P interacts with molecules associated with actin nucleation to inhibit reorganization of pseudopodium formation and cell motility.

【 授权许可】

Unknown