Antibacterial activity of peptides homologous to a loop region in human lactoferrin

FEBS Letters

Chapple, Daniel S.³  Evans, Robert W.²  Foxworthy, Morwenna³  Odell, Edward W.³  Sarra, Robert¹ 

[1] Department of Crystallography, Birkbeck College, Malet St, London WC1E 7HX, UK;Division of Biochemistry and Molecular Biology, UMDS Guy's Hospital, London SE1 9RT, UK;Department of Oral Medicine and Pathology, UMDS Guy's Hospital Dental School, London SE1 9RT, UK

关键词: Lactoferrin;    Lactoferricin;    Antimicrobial peptides;   

DOI  :  10.1016/0014-5793(96)00168-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human lactoferrin contains a 46 residue sequence named lactoferricin H thought to be responsible for its antimicrobial properties. Synthetic peptides HLT1, corresponding to the loop region of human lactoferricin (FQWQRNMRKVRGPPVS) and HLT2, corresponding to its charged portion (FQWQRNMRKVR), exerted significant antibacterial effects against E. coli serotype O111 strains NCTC 8007 and ML35. The corresponding sequences in native human lactoferrin were shown to adopt a charged helix and hydrophobic tall within the N-lobe remote from the iron binding site. Sequence similarities between lactoferricin and dermaseptin and magainins suggest that lactoferricin may act as an amphipathic alpha helix.

【 授权许可】

Unknown