Phase specific association of heterotrimeric GTP‐binding proteins with the actin‐based cytoskeleton during thrombin receptor‐mediated platelet activation

FEBS Letters

Sobue, Kenji¹  Ozawa, Kentaro¹  Takahashi, Masahiko¹ 

[1] Department of Neurochemistry and Neuropharmacology, Biomedical Research Center, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565, Japan

关键词: Platelet activation;    Cytoskeleton;    G protein;    p60c-src;    GPIIbIIIa;    G protein;    guanine nucleotide-binding protein;    GP glycoprotein;    DTT;    dithiothreitol;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(96)00162-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subcellular distribution of heterotrimeric GTP-binding proteins during thrombin receptor-mediated platelet activation was examined, revealing two phases of translocation to the cytoskeleton. A part of Gi2α and Gsα shows first phase translocation to the low-speed pellet (15000 × g pellet) within 1 min after activation, suggesting involvement in platelet shape change or granule secretion. In the second phase, Gi2α, Gsα, Gqα, and Gβ translocate to the low-speed pellet, depending on platelet aggregation. These translocation correlated with the reorganization of the actin-cytoskeleton and were inhibited by cytochalasin D. Reconstitution experiments also revealed that G proteins are associated with the actin-cytoskeleton during platelet activation.

【 授权许可】

Unknown