A link between ferritin gene expression and ribonucleotide reductase R2 protein, as demonstrated by retroviral vector mediated stable expression

FEBS Letters

Fan, Huizhou¹  Villegas, Cristy¹  Wright, Jim A.¹ 

[1] Manitoba Institute of Cell Biology and the University of Manitoba, 100 Olivia Street, Winnipeg, Manitoba, R3E 0V9, Canada

关键词: Ferritin;    DNA synthesis;    Ribonucleotide reductase;    Gene expression;   

DOI  :  10.1016/0014-5793(96)00143-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have constructed a retroviral expression vector for the mammalian ribonucleotide reductase R2 component. Stable infectants, which express a myc epitope tagged R2 protein from the vector cDNA were obtained and described for the first time. Cells containing the recombinant protein exhibited increased ribonucleotide reductase activity, and were resistant to the antitumour agent hydroxyurea, which targets the R2 component of ribonucleotide reductase. Furthermore, a direct link between ferritin gene expression and R2 protein was observed, since cells containing vector expressed recombinant R2 protein exhibited increased H-chain and L-chain ferritin gene expression.

【 授权许可】

Unknown