Rabbit translation elongation factor 1α stimulates the activity of homologous aminoacyl‐tRNA synthetase

FEBS Letters

El'Skaya, Anna V¹  Turkovskaya, Galina V¹  Shalak, Vyacheslav F¹  Budkevich, Tatyana V¹  Negrutskii, Boris S¹ 

[1] Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo str., Kiev 252143, Ukraine

关键词: Aminoacyl-tRNA synthetase;    Elongation factor;    Protein-protein interaction;    Channeling;    Rabbit liver;    EF-1α;    translation elongation factor 1α;    PheRS;    phenylalanyl-tRNA synthetase (EC 6.1.1.20);    TCA;    trichloroacetic acid;    DTT;    dithiothreitol;    SDS;    sodium dodecyl sulfate phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(96)00128-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Functional and structural sequestration of aminoacyl-tRNA has been recently found in eukaryotic cells and the aminoacyl-tRNA channeling has been suggested [B.S. Negrutskii et al., Proc. Natl. Acad. Sci. 91 (1994) 964–968], but molecular details and mechanism of the process remained unclear. In this paper we have verified a possible interaction between rabbit aminoacyl-tRNA synthetase and homologous translation elongation factor 1α (EF-1α), the proteins which may play a role of sequential components involved into the transfer of the aminoacyl-tRNA along the protein synthetic metabolic chain. The stimulation of the phenylalanyl-tRNA synthetase activity by EF-1α is found. The effect is shown to be specific towards the origin of tRNA and elongation factor molecules. The data obtained favor the direct transfer mechanism of the aminoacyl-tRNA channeling process during eukaryotic protein synthesis.

【 授权许可】

Unknown