Role of retinoic acid and oxidative stress in embryonic stem cell death and neuronal differentiation

FEBS Letters

Covarrubias, L.¹  Castro-Obregón, S.¹ 

[1] Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, UNAM, Apdo. Postal 510-3, Cuernavaca, Morelos 62271, México

关键词: Embryonic stem cell;    Cell death;    Neuronal differentiation;    Retinoic acid;    Oxidative stress;    ES;    embryonic stem;    2-ME;    2-mercaptoethanol;    ATRA;    all trans-retinoic acid;    EB;    embryoid bodies;    ROS;    reactive oxigen species;    SOD;    superoxide dismutase;    DCF;    dichlorofluorescein diacetate;    RA;    retinoic acid;   

DOI  :  10.1016/0014-5793(96)00088-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Embryonic stem (ES) cells are a suitable system to study events occurring during development. In the present work we show that the apoptotic program was activated in ES cells, either by simple removal of the reducing agent 2-mercapthoethanol (2-ME), or by addition of all trans-retinoic acid (ATRA) to embryoid bodies. In these two conditions, there was an increase in reactive oxygen species and antioxidants such as catalase, superoxide dismutase or phenol prevented ATRA-induced cell death. Neuronal differentiation was observed when undifferentiated ES cells were treated with ATRA in the absence of serum and the presence of 2-ME.

【 授权许可】

Unknown