Isolation and identification of glycated aminophospholipids from red cells and plasma of diabetic blood

FEBS Letters

Myher, J.J.²  Kamido, H.¹  Lewisa, G.³  Kuksis, A.²  Ravandi, A.²  Steiner, G.³  Marai, L.² 

[1] Department of Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan;Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada;Department of Medicine, University of Toronto, Toronto, Canada

关键词: Glucosylated aminophospholipid;    Glucose;    Phosphatidylethanolamine;    Phosphatidylserine;    Electrospray;    Thin-layer chromatography;    Liquid chromatography;    mass spectrometry;    Normal phase HPLC;   

DOI  :  10.1016/0014-5793(96)00064-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glycosylation is a major pathway for posttranslational modification of tissue protein and begins with non-enzymatic addition of carbohydrate to the primary amino groups. Excessive glycation of tissue protein has been implicated in the pathogenesis of diabetes and ageing. While glycation of aminophospholipids has also been postulated, glycated aminophospholipids have not been isolated. Using normal phase HPLC with on-line electrospray mass spectrometry we found glycated ethanolamine phospholipids to make up 10–16% of the total phosphatidylethanolamine (PE) of the red blood cells and plasma of the diabetic subjects. The corresponding values for glycated PE of control subjects were 1–2%.

【 授权许可】

Unknown