A specific Go heterotrimer couples somatostatin receptors to voltage‐gated calcium channels in RINm5F cells

FEBS Letters

Wittig, Burghardt²  Kalkbrenner, Frank¹  Degtiar, Vadim E.¹  Schultz, Günter¹ 

[1] Institut für Pharmakologie, Freie Universität Berlin, Thielallee 69-73, D-14195 Berlin, Germany;Institut für Molekularbiologie und Biochimie, Freie Universität Berlin, Arnimallee 22, D-14195 Berlin, Germany

关键词: Somatostatin receptor;    G-protein heterotrimer;    RINm5F microinjection;    Antisene oligonucleotide;   

DOI  :  10.1016/0014-5793(96)00047-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The peptide hormone somatostatin inhibits glucose-induced insulin secretion in the rat insulinoma RINm5F cells by inhibition of voltage-gated calcium channels. Here we used microinjection of antisene oligonucleotides directed against subtypes of G-protein subunits to determine the subunit composition involved in somatostatin-induced inhibition of voltage-gated calcium channels in RINmF5 cells. Injection of antisene oligonucleotides annealing to the respective mRNA of Gα_o2, Gβ₁ and Gγ₃ reduced the somatostatin-induced inhibition of calcium channels in these cells. Injection of antisene oligonucleotides directed against other G-protein subunits did not, suggesting that in RINm5F cells the somatostatin receptor couples to a G protein of Gα_o2β₁γ₃ composition. By using a selective agonist of type 2 somatostatin receptors (SSTR 2) NC 8–12, we identified this receptor as the subtype coupling to calcium channels in RINm5F cells.

【 授权许可】

Unknown