Double long‐chain amidine liposome‐mediated self replicating DNA transfection

FEBS Letters

El Ouahabi, A.²  Ruysschaert, J.M.²  Vandenbranden, M.²  Pector, V.²  Fuks, R.¹ 

[1] Laboratoire de Chimie Générale, Institut de Pharmacie, Université Libre de Bruxelles, Campus Plaine CP 206/4 B-1050 Brussels, Belgium;Laboratoire de Chimie-Physique des Macromolécules aux interfaces, Université Libre de Bruxelles, Campus Plaine CP 206/2, B-1050 Brussels, Belgium

关键词: Cationic liposome;    diC14-amidine;    mRNA transfection;    Self replicating recombinant mRNA;   

DOI  :  10.1016/0014-5793(96)00038-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We present experimental evidence that a complex made of a double long chain cationic amphiphile and recombinant mRNA facilitates the entry and expression of genetic material into cells. Combining the properties of the self replicating recombinant mRNA driven by the Semliki Forest Virus (SFV) replicon and the transfection potentialities of a new cationic amphiphile (N-t-butyl-N′-tetradecyl-3-tetradecylaminopropionamidine) yields a highly efficient mRNA transfection system conferring up to 100% infectivity. The preparation and characterization of the long chain amidine cationic amphiphile-mRNA complex as well as the influence of the diC14-amidine/RNA ratio on the infective activity are described.

【 授权许可】

Unknown