Cloning of rat GADD45 gene and induction analysis following ionizing radiation in vivo

FEBS Letters

Hughes, Paul E.¹  Schneider, Edward L.¹  Mori, Nozomu¹  Yoshida, Toru¹  Okazaki, Takashi¹ 

[1] Division of Neurogerontology, Ethel Percy Andrus Gerontology Center, University of Southern California, Los Angeles, CA 90089, USA

关键词: Gene cloning;    DNA damage inducible gene;    Ionizing radiation;    Induction (in vivo);    GADD45;    growth arrest and DNA damage inducible (protein);    CHO;    chinese hamster ovary;    AT;    ataxia telangiectasia;    MMS;    methyl methanesulfonate;    PCR;    polymerase chain reaction;    PGK;    phosphoglycerate kinase;    PCNA;    proliferating cell nuclear antigen;   

DOI  :  10.1016/0014-5793(96)00012-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene encoded GADD45 was isolated from rat and revealed four exons along with a p53 binding consensus sequence and a putative AP-1 site in the third intron. This suggests that the rat GADD45 gene is also involved in the p53 signal pathway related to the G₁ cell cycle checkpoint. The rat GADD45 mRNA was induced within 30 min in liver and increased as a function of γ-irradiation. We found that mRNA expression differed substantially in a variety of tissues (brain, liver, kidney, and spleen). The finding of in vivo induction of GADD45 gene may provide insight into the role of GADD45 gene in DNA repair.

【 授权许可】

Unknown