Identification of disulfide bonds in the ninth component (C9) of human complement

FEBS Letters

Lengweiler, Stephan¹  Rickli, Egon E.¹  Schaller, Johann¹ 

[1] Institute of Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland

关键词: Complement;    C9;    Disulfide bridge;    Terminal complement component;    MAC;    membrane attack complex;    TSP I;    thrombospondin type I repeat;    LDL A;    low density lipoprotein receptor class A module;    LDL B;    low density lipoprotein receptor class B module;    EGF;    epidermal growth factor;    RP-HPLC;    reversed phase high performance liquid chromatography;    SDS-PAGE;    polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate;    BNPS-skatole;    2-(2-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine;    SBD-F;    ammonium 7-fluorobenz-2-oxa-1;    3-diazole-4-sulfonate;    DTT;    dithiothreitol;    PTH;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(95)01541-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

C9 is the most abundant protein of the membrane attack complex of complement. By means of limited proteolysis, different chromatographic techniques, a thiol-specific fluorescence assay, amino acid analysis, and Edman degradation, 9 out of 12 disulfide bridges are definitely assigned (Cys²²Cys⁵⁷, Cys³³Cys³⁶, Cys⁶⁷Cys⁷³, Cys¹²¹Cys¹⁶⁰, Cys²³³Cys²³⁴, Cys³⁵⁹Cys³⁸⁴, Cys⁴⁸⁹Cys⁵⁰⁵, Cys⁴⁹²Cys⁵⁰⁷, Cys⁵⁰⁹Cys⁵¹⁸). Weaker evidence permits to reduce the number of possible configurations for the remaining 3 cystines (Cys⁸⁰Cys⁹¹, Cys⁸⁶Cys¹⁰⁴, Cys⁹⁸Cys¹¹³, or Cys⁸⁰Cys⁹¹, Cys⁸⁶Cys¹¹³, Cys⁹⁸Cys¹⁰⁴). These findings are discussed in comparison with the strongly related components C6, C7, C8α, and C8β.

【 授权许可】

Unknown