Accumulation of nitrotyrosine on the SERCA2a isoform of SR Ca‐ATPase of rat skeletal muscle during aging: a peroxynitrite‐mediated process?

FEBS Letters

Schöneich, Christian²  Hühmer, Andreas F.R.²  Bigelow, Diana J.¹  Viner, Rosa I.²  Ferrington, Deborah A.¹ 

[1] Department of Biochemistry, University of Kansas, Lawrence, KS 66045, USA;Department of Pharmaceutical Chemistry, University of Kansas, Lawrence KS 66045, USA

关键词: Peroxynitrite;    Nitric oxide;    Nitrotyrosine;    Reactive oxygen species;    Aging;    SR Ca-ATPase;    DEA/NO;    diethylamine/nitric oxide complex sodium salt;    DTT;    dithiothreitol;    MOPS;    3-(N-morpholino)propanesulfonic acid;    SDS-PAGE;    sodium dodecylsulfate-polyacrylamide gel electrophoresis;    SR;    sarcoplasmic reticulum;   

DOI  :  10.1016/0014-5793(95)01530-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The SR Ca-ATPase in skeletal muscle SR vesicles isolated from young adult (5 months) and aged (28 months) rats was analyzed for nitrotyrosine. Only the SERCA2a isoform contained significant amounts with approximately one and four nitrotyrosine residues per young and old Ca-ATPase, respectively. The in vitro exposure of SR vesicles of young rats to peroxynitrite yielded selective nitration of the SERCA2a Ca-ATPase even in the presence of excess SERCA1a. No nitration was observed during the exposure of SR vesicles to nitric oxide in the presence of O₂. These data suggest the in vivo presence of peroxynitrite in skeletal muscle. The greater nitrotyrosine content of SERCA2a from aged tissue imolies an age-associated increase in susceptibility to oxidation by this species.

【 授权许可】

Unknown