Murine and human mast cell express acetylcholinesterase

FEBS Letters

Soreq, Hermona¹  Razin, Ehud²  Nechushtan, Hovav²  Kuperstein, Valentina²  Tshori, Sagi² 

[1] Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91120, Israel;Department of Biochemistry, Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, PO Box 12272, Jerusalem 91120, Israel

关键词: AChE;    Mast cell;    Single-cell RT-PCR;    Human precursor cell;    Alternative splicing;    AChE;    acetylcholinesterase;    BuChE;    butyrylcholinesterase;    ACh;    acetylcholine;    ATCh;    acetylthiocholine;   

DOI  :  10.1016/0014-5793(95)01466-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of catalytically active protein was detected in a murine mast cell line. The primary type of AChE mRNA produced by these cells was found to be the brain and muscle type by PCR amplification of alternative exons from the 3′ of mast cells AChE cDNA. AChE was further found to be expressed in the HMC-1 the human mast cell precursor line. Furthermore, utilizing the single cell RT-PCR method we detected AChE mRNA expression in FcϵRI-positive single cells derived from human colonic mucosal biopsies. Our findings predict the involvement of mast cell AChE in neuronal-mast cell interactions.

【 授权许可】

Unknown