The modulation of the rate of inactivation of the mKv1.1 K+ channel by the β subunit, Kvβ1 and lack of effect of a Kvβ1 N‐terminal peptide

FEBS Letters

Schecter, L.E.¹  Owen, David G.²  Nawoschik, S.P.¹  Cockett, Mark I.¹  Stephens, Gary J.² 

[1] Wyeth-Ayerst Research, Princeton, NJ, USA;Electrophysiology Laboratory, Wyeth Research - UK, Huntercombe Lane South, Taplow, Berkshire, SL6 0PH, UK

关键词: Cloned K+ channel;    Subunit;    Inactivation;    Patch clamp;    CHO cell;   

DOI  :  10.1016/0014-5793(95)01469-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The coexpression of the rat Kvβ1 subunit with the mouse Kv1.1 (mKv1.1) K⁺ channel in Chinese hamster ovary cells caused an increase in the rate of inactivation of whole-cell current. Current decayed in a bi-exponential fashion with a fast voltage-dependent and a slower voltage-independent component. The inactivating current component accounted for around 40% of the total outward current. In contrast to previous studies using K⁺ channel α subunits, peptides based on the N-terminal of the Kvβ1 subunit were unable to mimic the action of the entire subunit. The findings indicate differences between the inactivation induced by the Kvβ1 subunit and the N-type inactivation mechanism associated with certain rapidly-inactivating cloned K⁺ channel α subunits.

【 授权许可】

Unknown