FEBS Letters | |
Functional expression of bacteriorhodopsin in oocytes allows direct measurement of voltage dependence of light induced H+ pumping | |
Büldt, Georg2  Möckel, Bettina2  Bamberg, Ernst1  Nagel, Georg1  | |
[1] Max-Planck-Institut für Biophysik, Kennedy-Allee 70, D-60596 Frankfurt, Germany;IBI-2, Forschungszentrum Jülich, D-52425 Jülich, Germany | |
关键词: Bacteriorhodopsin; H+-pump; Voltage dependence; Xenopus oocyte; BLM; black lipid membrane; BR; bacteriorhodopsin; bop; bacterio-opsin; EGTA; ethylene glycol-bis(β-aminoethyl ether) N; N; N′; N′-tetraacetic acid; HEPES; N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfo nic acid]; MOPS; 3-[N-morpholino]propanesulfonic acid; NMG; TEA; tetraethylammonium; V h; holding potential; | |
DOI : 10.1016/0014-5793(95)01356-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We report on the first successful expression of the light driven H+ pump, bacteriorhodopsin, into the plasma membrane of oocytes from Xenopus laevis. The light induced photocurrents which reflect the pumping of H+ by BR were analysed under voltage clamp conditions. At least 100 active BR molecules per μm2 were expressed in the plasma membrane so that both the voltage clamp and giant patch clamp method could be applied. We show that H+ pumping by BR is modulated by the membrane potential, i.e. the pump current shows strong voltage dependence in the range measured between −165 mV to +60 mV.
【 授权许可】
Unknown
【 预 览 】
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