Immunological evidence that HMG‐CoA reductase kinase‐A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases

FEBS Letters

Barker, Jacqueline¹  Hardie, D.Grahame²  Halford, Nigel G.¹  Ball, Kathryn L.² 

[1] IACR, Long Ashton Research Station, Department of Agricultural Sciences, Bristol University, Bristol BS18 9AF, UK;Department of Biochemistry, The University, Dundee DD1 4HN, Scotland, UK

关键词: HMG-CoA reductase kinase;    AMP-activated protein kinase;    SNF1;    RKIN1;    BKIN12;    AKIN10;    AMPK;    AMP-activated protein kinase;    HMG-;    3-hydroxy-3-methylglutaryl-;    HRK-A;    HMG-CoA reductase kinase-A;    HRK-B;    HMG-CoA reductase kinase-B;    SNF1;    sucrose non-fermenting-1;    ELISA;    enzyme-linked immunoassay;    PBS;    phosphate-buffered saline;    PBST;    phosphate-buffered saline containing Tween 20;   

DOI  :  10.1016/0014-5793(95)01343-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots. HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces cereviseae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.

【 授权许可】

Unknown