Stimulatory effect of ouabain on VCAM‐1 and iNOS expression in murine endothelial cells: involvement of NF‐κB

FEBS Letters

Bereta, Joanna¹  Bereta, Michał¹  Cohen, Marion C.² 

[1] Institute of Molecular Biology, Jagiellonian University, Al. Mickiewicza 3, 31–120 Kraków, Poland;Department of Anatomy, Cell Biology and Injury Sciences, New Jersey Medical School, Newark, NJ, USA

关键词: Ouabain;    Na+/K+-ATPase;    VCAM-1;    iNOS;    NF-κB;    PBS;    phosphate-buffered saline;    FCS;    fetal calf serum;    IFN-γ;    interferon-γ;    IL-1;    interleukin 1;    iNOS;    inducible nitric oxide synthase;    LPS;    lipopolysaccharide;    MME;    murine microvascular endothelial cells;    Na+/K+-ATPase;    Na+ and K+-dependent adenosine triphosphatase;    NF-κB;    nuclear factor κB;    TNF;    tumor necrosis factor-α;    VCAM-1;    vascular cell adhesion molecule-1;   

DOI  :  10.1016/0014-5793(95)01301-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endothelial cells play a pivotal role in the development of atherosclerosis. An ‘activated’ phenotype of these cells is manifested by signal transduction-dependent expression of genes encoding cytokines, pro- and anticoagulant factors, and cell adhesion molecules. In the current study we examined the effect of ouabain, an inhibitor of Na⁺/K⁺-ATPase, on the process of endothelial cell activation. We demonstrated that ouabain was able to stimulate VCAM-1 expression and potentiate the effect of IFN-γ on this process. Moreover, ouabain provided a complementary signal for either TNF or IFN-γ in inducing iNOS expression. Our data also show, for the first time, that inhibition of Na⁺/K⁺-ATPase led to activation of the transcription factor, NF-κB, which may provide an explanation for the effects of ouabain on endothelial cells.

【 授权许可】

Unknown