Functional expression of an alpha anti‐insect scorpion neurotoxin in insect cells and lepidopterous larvae

FEBS Letters

Rivkin, Hadasah²  Gurevitz, Michael¹  Zlotkin, Eliahu³  Chejanovsky, Nor²  Zilberberg, Noam¹ 

[1] Department of Botany, Tel-Aviv University, Tel Aviv 69978, Israel;Institute of Plant Protection, ARO, Volcani Center, Bet Dagan 50250, Israel;Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

关键词: LqhαIT (Leiurus quinquestriatus hebraeus) anti-insect toxin;    Baculovirus;    Insect cells;    Lepidopterous larvae;   

DOI  :  10.1016/0014-5793(95)01263-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Leiurus quinquestriatus hebraeus alpha anti-insect toxin (LqhαIT) cDNA was engineered into the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant virus secreted a functional LqhαIT polypeptide. Spodoptera littoralis and Heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. This recombinant virus showed enhanced insecticidal potency against H. armigera larvae compared with wild type AcNPV. The present expression system will facilitate: (1) the future elucidation of structural elements involved in its prominent anti-insect toxicity; and (2) the future design of genetically modified alpha toxins with improved anti-insect selectivity.

【 授权许可】

Unknown