Automation of micro‐preparation and enzymatic cleavage of gel electrophoretically separated proteins

FEBS Letters

Mann, Matthias¹  Houthaeve, Tony¹  Ashman, Keith¹  Gausepohl, Heinrich² 

[1] Protein and Peptide Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69012 Heidelberg, Germany;ABIMED Gmbh, Raiffeisenstrasse 3, 40736 Langenfeld, Germany

关键词: Automation;    In-gel proteolysis;    Internal protein sequence analysis;    Micro-characterization;    Mass spectrometry;    Protein identification;   

DOI  :  10.1016/0014-5793(95)01242-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To achieve high throughput, protein microcharacterization sample preparation must be automated. We describe a cartesian robot capable of processing 32 protein samples in parallel. The system is based on specially designed flow-through reactors for contamination-free reagent delivery and removal. Washing of excised gel pieces, reduction and alkylation, proteolytic cleavage and peptide extraction are performed in these reactors. Compatibility of the system with HPLC peptide separation and Edman degradation as well as with laser desorption mass spectrometry of the unseparated mixture is demonstrated. This is the first report describing automated preparation and processing of multiple protein samples.

【 授权许可】

Unknown