β‐Glucosylarginine: a new glucose‐protein bond in a self‐glucosylating protein from sweet corn

FEBS Letters

Serwe, Maria³  Lomako, Wieslawa M.²  Singh, David G.²  Meyer, Helmut E.³  Lomako, Joseph²  Metzger, Jörg W.¹  Whelan, William J.² 

[1] Institut für Organische Chemie, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 18, D-72076 Tübingen, Germany;Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, (M823), PO Box 016129, Miami, FL 33101, USA;Institut für Physiologische Chemie, Ruhr-Universität Bochum, Universitätsstrasse 150, D-44780 Bochum, Germany

关键词: Amylogenin;    β-Glucosylarginine;    Self-glucosylating protein;    Autoglucosylation;   

DOI  :  10.1016/0014-5793(95)01247-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the search for a protein primer for starch synthesis, an autocatalytic self-glucosylating protein has been isolated from sweet corn. Several tryptic peptides were obtained from the [¹⁴C]glucosylated protein and were sequenced, corresponding to over 40% of the estimated total sequence (molecular mass 42 kDa). There is no homology with the amino acid sequence of the autocatalytic glycogen primer, glycogenin, nor in respect of the nature of the union between the autocatalytically added glucose and the protein, which, in the case of the corn protein, now named amylogenin, is a novel glucose-protein bond, a single β-glucose residue joined to an arginine residue.

【 授权许可】

Unknown