Molecular characterization of two forms of nontoxic‐nonhemagglutinin components of Clostridium botulinum type A progenitor toxins

FEBS Letters

Inoue, Kaoru¹  Oguma, Keiji¹  Nakajima, Hiroshi¹  Fujinaga, Yukako¹  Kumon, Hiromi²  Fujita, Ryuji¹ 

[1] Department of Bacteriology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700, Japan;Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700, Japan

关键词: Clostridium botulinum;    Botulinum toxin;    Neurotoxin;    Progenitor toxin;    Nontoxic-nonhemagglutinin;    PCR;   

DOI  :  10.1016/0014-5793(95)01241-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The entire sequences of the type A nontoxic-nonhemagglutinin gene and an adjacent open reading frame designated as orf 22-a, which are located between the neurotoxin and the HA-35 genes were determined. SDS-PAGE and N-terminal amino acid sequence analyses of the purified type A progenitor toxins (12S, 16S and 19S) indicate that the nontoxic-nonhemagglutinins of 16S and 19S are single peptides of approximately 120k, but that of 12S has a cleavage at the site between Pro-144 and Phe-145 of this protein.

【 授权许可】

Unknown