Agonist regulation of the expression of the delta opioid receptor in NG108‐15 cells

FEBS Letters

Chin, Hemin¹  Kim, Dong Sun²  Klee, Werner A.² 

[1] Laboratory of Neurochemistry, NINDS, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA;Laboratory of Molecular Biology, NIMH, Bldg. 36, Rm. 1B08, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA

关键词: δ-Opioid receptor;    Opiate;    Binding activity;    Transcription;    Down-regulation;   

DOI  :  10.1016/0014-5793(95)01233-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of neuronal cells to the chronic presence of opiates leads to a complex series of biochemical events which reflect the changes that result in tolerance and dependence in animals. To achieve a better understanding of the molecular mechanisms underlying these processes, we have examined the effect of agonist efficacy on the regulation of the δ-opioid receptor mRNA in NG108-15 cells. Incubation with various opiates decreased receptor numbers in the order of their efficacy. Northern blot analysis showed that there are 4 size classes of mRNA coding for the δ-opioid receptor in NG108-15 cells even though only one known protein species is found. Moreover, the amount of each transcript is coordinately decreased by long-term etorphine treatment, but not necessarily to the same extent. The etorphine-induced decrease in receptor mRNA was found to be slow in onset, whereas a much more rapid loss of receptor number was observed. This disparity suggests that the down-regulation induced by etorphine can occur both at the levels of receptor protein modification and receptor gene expression, and that the mechanisms of the two processes may be different.

【 授权许可】

Unknown