Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase

FEBS Letters

Weser, Ulrich¹  Kaup, Yoka¹  Etspüler, Hedwig¹ 

[1] Anorganische Biochemie, Physiologisch Chemisches Institut der Universität Tübingen, Hoppe-Seyler-Str. 4, D-72076 Tübingen, Germany

关键词: Bone alkaline phosphatase;    Ancient enzyme;    ELISA;    Monoclonal antibody;    Egyptian mummy;   

DOI  :  10.1016/0014-5793(95)01227-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Structurally intact and functionally active human bone alkaline phosphatase was isolated from clavicle fragments of IDU, an Egyptian mummy of the Old Kingdom (2150 ± 50 BC). Both anion exchange and affinity chromatographies were employed to optimise the preparation of the ancient enzyme resulting in a specific activity of 180 ± 30 mU/mg. The intactness of the bone enzyme fractions of the wheat-germ lectin affinity chromatography was successfully demonstrated in an ELISA using the monoclonal antibody BAP A. Fortunately, the mummified bone was not contaminated by fungi or bacteria.

【 授权许可】

Unknown