Predicted structure and possible ionmotive mechanism of the sodium‐linked NADH‐ubiquinone oxidoreductase of Vibrio alginolyticus

FEBS Letters

Rich, Peter R.¹  Meunier, Brigitte¹  Ward, F.Bruce² 

[1] Glynn Research Foundation, Bodmin, Cornwall PL30 4AU, UK;Institute for Cell and Molecular Biology, Edinburgh University, Edinburgh, UK

关键词: NADH-ubiquinone oxidoreductase;    NADH dehydrogenase;    Sodium ion translocation;   

DOI  :  10.1016/0014-5793(95)01164-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two groups have now published sequences of the six genes contained in the operon coding for the sodium-linked NADH-ubiquinone oxidoreductase of Vibrio alginolyticus. Sequence analyses indicate that this enzyme is unrelated to other known respiratory NADH dehydrogenases. A search for cofactor motifs suggests that the enzyme contains only one FAD, a ferredoxin-type iron sulphur centre, and the NADH-binding site. These are all located on NqrF, a subunit that can be recognized as a new member of a large diverse family of NAD(P)H-oxidizing flavoenzymes. A possible model of ion-coupling is presented, based upon this new information.

【 授权许可】

Unknown