Nitrogen oxide‐induced autoprotection in isolated rat hepatocytes

FEBS Letters

Kim, Young-Myeong¹  Bergonia, Hector¹  Lancaster, Jack R.² 

[1] Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA;Departments of Physiology and Medicine, LSU Medical Center, 1901 Perdido Street, New Orleans, LA 70112, USA

关键词: Nitric oxide;    Nitrogen oxide;    Rat hepatocyte;    Reactive oxygen species;   

DOI  :  10.1016/0014-5793(95)01115-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pretreatment of rat hepatocytes with low-dose nitrogen oxide (addition of SNAP in vitro or induction of nitric oxide synthase in vitro or in vivo) imparts resistance to killing and decrease in aconitase and mitochondrial electron transfer from a second exposure to a higher dose of SNAP. Induction of this resistance is prevented by cycloheximide, indicating upregulation of protective protein(s). Ferritin levels are increased as are nonheme iron-NO EPR signals. Tin-protoporphyrin (SnPP) prevents protection, suggesting involvement of hsp32 (heme oxygenase) and/or guanylyl cyclase (GC). Cross-resistance to H₂O₂ killing is also observed, which is also prevented by cycloheximide and SnPP. Thus, hepatocytes possess inducible protective mechanisms against nitrogen oxide and reactive oxygen toxicity.

【 授权许可】

Unknown