Rapid purification of malate synthase from cotyledons of Brassica napus L

FEBS Letters

Hoppe, Andreas¹  Theimer, Roland R.¹ 

[1] Physiologische Chemie der Pflanzen, FB 9, Bergische Universität GH Wuppertal, Gauss-Str. 20, D-42097 Wuppertal, Germany

关键词: Malate synthase;    Glyoxysome;    Rape;    Brassica napus L;   

DOI  :  10.1016/0014-5793(95)01114-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A rapid and efficient method for the purification of malate synthase, an enzyme uniquely confined to glyoxysomes, from cotyledons of Brassica napus L. has been developed. The two step purification procedure is based on the consequent utilization of the tendency of malate synthase to form high molecular weight aggregates. Malate synthase was purified 75-fold to apparent homogeneity with a specific activity of 180 nkat/mg protein. The estimated molecular weight of malate synthase subunits was 63 kDa. Polyclonal antibodies raised against malate synthase in rabbits detect on Western blots only one single polypeptide with an identical molecular weight.

【 授权许可】

Unknown