Reconstitution photoactive yellow protein from apoprotein and p‐coumaric acid derivatives

FEBS Letters

Imamoto, Yasushi¹  Tokunaga, Fumio¹  Kataoka, Mikio¹  Ito, Takeshi¹ 

[1] Department of Earth and Space Science, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan

关键词: Photoactive yellow protein;    Photoreceptor protein;    p-Coumaric acid;    Chromophore;    Reconstitution;    Ectothiorhodospira halophila;   

DOI  :  10.1016/0014-5793(95)01096-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report reconstitution of photoactive yellow protein (PYP) from apoPYP and p-coumaric acid derivatives. The addition of p-coumaric acid to the apoPYP sample did not result in the recovery of PYP. In contrast, yellow products were obtained by the addition of p-coumaryl thiophenyl ester or p-coumaric anhydride to the apoPYP sample, the absorption spectra of which were indistinguishable from the spectrum of intact PYP. Our findings provide strong evidence that PYP has the p-coumaryl chromophore. This reconstitution technique opens the way for further biophysical studies of PYP using artificial chromophore analogs.

【 授权许可】

Unknown