A new mutation in the pufL gene responsible for the terbutryn resistance phenotype in Rubrivivax gelatinosus

FEBS Letters

Ouchane, S.¹  Picaud, M.¹  Astier, C.¹ 

[1] CNRS, Centre de Génétique Moléculaire, Photosynthèse Bactérienne, bât 24, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cédex, France

关键词: Photosynthetic bacterium;    Herbicide;    Herbicide mutant;    Cross-resistance;    Reaction center;   

DOI  :  10.1016/0014-5793(95)01055-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rubrivivax gelatinosus is a facultative phototrophic non-sulfur bacterium belonging to the β subclass of the purple bacteria. A terbutryn-resistant mutant of R. gelatinosus has been isolated and characterized. Increased resistance levels to terbutryn (300-fold), atrazine (6-fold) and o-phenanthroline (3-fold) were observed for the mutant compared with wild type. Sequence analysis of the mutant revealed a new mutation in the pufL gene coding for the L subunit of the reaction centre (RC) at codon 192 leading to an amino-acid substitution from Gly in the wild type to Asp in the mutant. This substitution is located in the D helix of the L subunit, suggesting an interaction between terbutryn and this part of the polypeptide in the RC of R. gelatinosus. This is the first report of a mutation leading to herbicide resistance and affecting the D helix in purple bacteria. Furthermore R. gelatinosus wild type is highly sensitive to o-phenanthroline compared with other purple bacteria (Rhodobacter capsulatus and Rhodobacter sphaeroides). Sequence comparison of the L subunit from six purple bacteria in which o-phenanthroline sensitivity was measured suggests that Ser^L226 might be responsible for this phenotype.

【 授权许可】

Unknown