Crystallization of threonyl‐tRNA synthetase from Thermus thermophilus and preliminary crystallographic data

FEBS Letters

Mitschler, A.¹  Cura, V.¹  Kern, D.²  Moras, D.¹ 

[1] UPR 9004 de Biologie Structurale, IGBMC, CNRS/INSERM/ULP, 1 rue L. Fries / BP 163, F-67404 Illkirch Cédex, France;UPR 9002 SMBMR, IBMC du CNRS, 15 rue R. Descartes, F-67084 Strasbourg Cédex, France

关键词: Aminoacyl-tRNA synthetase;    Thermus thermophilus;    Crystallization;    tRNA aminoacylation;   

DOI  :  10.1016/0014-5793(95)01089-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Threonyl-tRNA synthetase from Thermus thermophilus (ttTRS) has been overproduced in Escherichia coli, purified and crystallized in solutions containing ammonium sulfate and glycerol. The crystals grew in the orthorhombic space group C222₁ with unit cell dimensions a = 119.5 Å, b = 120.0 Å, c = 317.5 Å. The asymmetric unit is constituted of two monomers and the crystals contain 66% solvent. This paper reports the first crystals of ttTRS and preliminary crystallographic results since the presumed crystals of ttTRS described in a previous paper [1] were crystals of aspartyl-tRNA synthetase [2].

【 授权许可】

Unknown