Cloning and sequencing of a cDNA encoding rat d‐dopachrome tautomerase

FEBS Letters

Panagopoulos, Ioannis²  Zhang, Miao²  Hindemith, Annika¹  Åman, Pierre²  Grubb, Anders³  Rorsman, Hans⁴  Rosengren, Evald¹ 

[1] Department of Pharmacology, University of Lund, S-221 85 Lund, Sweden;Department of Clinical Genetics, University Hospital, S-221 85 Lund, Sweden;Department of Clinical Chemistry, University Hospital, S-221 85 Lund, Sweden;Department of Dermatology, University Hospital, S-221 85 Lund, Sweden

关键词: d-Dopachrome tautomerase;    Rat liver;    PCR;    cDNA cloning;    Macrophage migration;   

DOI  :  10.1016/0014-5793(95)01041-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An enzyme which converts d-dopachrome into 5,6-dihydroxyindole has recently been isolated from rat liver. Enzymatic d-dopachrome conversion has been observed in extracts from all tissues examined of several species, including man. We have now cloned and sequenced a 628 bp long cDNA encoding the enzyme provisionally called d-dopachrome tautomerase. The cDNA was isolated by 3′ and 5′ rapid amplification and cloning of cDNA ends (RACE) from rat liver cells using degenerate oligonucleotide primers, deduced from the N-terminal peptide sequence of d-dopachrome tautomerase. The cDNA contains an open reading frame encoding 118 amino acids. Edman degradation of intact and of trypsin degraded d-dopachrome tautomerase fragments gave information on and corroborated 67% of the deduced protein sequence. A homology search in the EST database found a human cDNA encoding a peptide sharing 66% homology with the rat enzyme. The rat d-dopachrome tautomerase shares 27% homology with the rat macrophage migration inhibitory factor (MIF).

【 授权许可】

Unknown