Voltage‐gated K+ currents of mouse dendritic cells

FEBS Letters

Eder, Claudia²  Fischer, Hans-Georg¹ 

[1] Institut für Medizinische Mikrobiologie und Virologie, Heinrich-Heine-Universität, Universitätsstr. 1, D-40225 Düsseldorf, Germany;Abt. Neurophysiologie, Institut für Physiologie der Charité, Humboldt Universität zu Berlin, Tucholsky Str. 2, D-10117 Berlin, Germany

关键词: Dendritic cell;    MHC class II;    Antigen presentation;    Patch clamp;    Potassium current;    DC;    dendritic cells;    FCS;    fetal calf serum;    GM-CSF;    granulocyte/macrophage colony-stimulating factor;    ICAM;    intercellular adhesion molecule;    IIR;    inward rectifying potassium current;    IK;    outward potassium current;    IK′;    frequency-independent IK;    IMDM;    Iscove's modified Dulbecco's medium;    LFA;    lymphocyte function associated antigen;    mAb;    monoclonal antibody;    MHC;    major histocompatibility complex;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(95)01029-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dendritic cells (DC) were enriched from murine spleen by exploiting their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofunctional characteristics of DC. Cells of typical dendritic shape were electrophysiologically analysed using the whole cell configuration of the patch clamp technique. All 26 cells expressed only outward K⁺ currents comparable to those detected in cytokine-activated microglia. Co-purified splenic macrophages, in contrast, displayed an inward rectifying K⁺ current.

【 授权许可】

Unknown