Shear stress induces expression of CNP gene in human endothelial cells

FEBS Letters

Fujiwara, Yoshiyuki¹  Kawasaki, Tomio¹  Ohnishi, Tadashi¹  Monden, Morito¹  Kambayashi, Jun-ichi¹  Okahara, Kazuhiro¹ 

[1] Department of Surgery II, Osaka University Medical School, 2-2, Yamadaoka, Suita, Osaka 565, Japan

关键词: Shear stress;    C-type natriuretic peptide;    Human endothelial cell;    mRNA;    CNP;    C-type natriuretic peptide;    RT-PCR;    reverse transcription-polymerase chain reaction;    ANP;    atrium natriuretic peptide;    BNP;    brain natriuretic peptide;    cGMP;    guanosine 3′:5′-cyclic monophosphate;    TGFβ;    transforming growth factor-beta;    HUVEC;    human umbilical vein endothelial cell;    FBS;    fetal bovine serum;    FGF;    fibroblast growth factor;    PBS;    phosphate buffered saline;    GAPDH;    glyceraldehyde 3-phosphate dehydrogenase;    SDS;    sodium dodecyl sulfate;    ICAM;    intracellular adhesion molecule;    cNOS;    constitutive nitric oxide synthase;    SSRE;    shear stress responsive element;   

DOI  :  10.1016/0014-5793(95)01027-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To elucidate the effect of blood flow on gene transcription of C-type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone-plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription-polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm² at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells.

【 授权许可】

Unknown