Human pancreatic phospholipase A2 stimulates the growth of human pancreatic cancer cell line

FEBS Letters

Sugiyama, Masanori¹  Itoh, Masaki²  Hirata, Manabu²  Hanada, Keiji²  Kajiyama, Goro²  Kinoshita, Eiji¹ 

[1] Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734, Japan;First Department of Internal Medicine, Hiroshima University School of Medicine, 1-2-3, Kasumi, Minami-ku, Hiroshima 734, Japan

关键词: Pancreatic phospholipase A2 (PLA2);    Pancreatic cancer cell line;    Proliferation;    Receptor for PLA2;    h;    human;    PLA2;    phospholipase A2;    PAGE;    polyacrylamide gel electrophoresis;    SDS;    sodium dodecyl sulphate;   

DOI  :  10.1016/0014-5793(95)01005-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phospholipase A₂ (PLA₂) from human pancreas, designated hPLA₂-I, functions as a digestive enzyme. Interestingly, the present study demonstrated that the mature form of hPLA₂-I stimulated the growth of a human pancreatic cancer cell line MIAPaCa-2, whereas the pro-form was ineffective. PLA₂s from Laticauda semifasciata fraction I, Crotalus adamanteus venom, Streptomyces violaceoruber and bee venom, showed no proliferative effect to the growth of MIAPaCa-2. The Scatchard plot analysis revealed that the MIAPaCa-2 cell had a specific binding site for the mature hPLA₂-I. The equilibrium binding constant (K _d) and the maximum binding capacity (B _max) were 2.6 nM and 0.4 fmol/10⁶ cells, respectively. These results suggest that the mature hPLA₂-I, but not the pro-form, may function as a growth factor of pancreas carcinoma via the specific binding site.

【 授权许可】

Unknown