Localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells

FEBS Letters

Beattie, John²  Glover, L.Anne¹  Hesketh, John²  Mahon, Patrick² 

[1] Department of Molecular and Cell Biology, Marishal College, Aberdeen AB9 1AS, Scotland, UK;Division of Biochemical Sciences, Rowett Research Institute, Greenburn Rd., Bucksburn, Aberdeen AB2 9SB, Scotland, UK

关键词: Cytoskeleton;    Polysome;    mRNA;    Metallothionein;    Metal;    Compartmentation;   

DOI  :  10.1016/0014-5793(95)01000-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells was investigated using two approaches, namely Northern hybridisation of total RNA extracted from free, cytoskeletal-bound and membrane-bound polysomes isolated by a sequential detergent/salt extraction procedure and in situ hybridisation. The cytoskeletal-bound polysomes were enriched in metallothionein-I (MT-I) and c-myc mRNAs but showed a significantly lower enrichment in MT-II mRNA. These findings indicate that the MT-I mRNA is localised to the cytoskeleton during translation. In situ hybridisation using a biotin-labelled oligonucleotide probe revealed a predominantly perinuclear localisation for the MT-I mRNA.

【 授权许可】

Unknown