Identification of the human mitochondrial protein import receptor, huMas20p. Complementation of Δmas20 in yeast

FEBS Letters

Swie Goping, Ing¹  Shore, Gordon C.¹  Millar, Douglas G.¹ 

[1] Department of Biochemistry, McIntyre Medical Sciences Building, McGill University, Montreal, Quebec, H3G 1 Y6, Canada

关键词: Mitochondrion;    Import receptor;    Protein targeting;   

DOI  :  10.1016/0014-5793(95)01010-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human homolog of the S. cerevisiaelN. crassa mitochondrial protein import receptor, Mas20p/MOM19, has been identified and characterized. Sequence similarities between these three proteins is most pronounced within the NH₂-terminal third of the molecules. However, the mammalian protein exhibits only weak homology to the tetratricopeptide repeat B domain that is found in Mas20p/MOM19. huMas20p is targeted and inserted into the outer membrane of isolated rat heart mitochondria, in the N_in-C_cyto orientation. Antibodies directed against the soluble portion of huMas20p inhibited in vitro mitochondrial import of a diverse set of precursor proteins (including inner membrane uncoupling protein), but failed to block import of a fusion protein bearing the signal-anchor sequence of Mas20p itself. Finally, expression of huMAS20 complemented the respiratory defect of Δmas20 yeast cells. Together, these results demonstrate that huMAS20p is a component of the mammalian import apparatus.

【 授权许可】

Unknown