A novel tau‐tubulin kinase from bovine brain

FEBS Letters

Omori, Akira¹  Takahashi, Miho¹  Sato, Kazuki¹  Tomizawa, Kayoko¹  Ohtake, Atsuko¹ 

[1] Mitsubishi Kasei Institute of Life Sciences (Project 2), 11 Minamiooya, Machida-shi, Tokyo 194, Japan

关键词: Protein kinase;    Tau;    Tubulin;    Ser-Arg motif;    Bovine brain;    PHF;    paired helical filaments;    TTK;    tau-tubulin kinase;    TPK;    tau protein kinase;    CK;    casein kinase;    MAP;    microtuble-associated protein;    SDS;    sodium dodecyl sulfate;    PTH;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(95)00955-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

During purification of tau protein kinase I and II from the bovine brain extract, a new tau protein kinase was detected and purified with phosphocellulose, gel filtration, S-Sepharose and AF-Heparin column chromatography. The molecular mass of the enzyme was determined to be 32 kDa by gel filtration and activity staining on SDS-PAGE. The enzyme is a Ser/Thr protein kinase phosphorylating tau, β-tubulin, MAP2 and α-casein. Employing many synthetic peptides, the recognition site of this enzyme appears to be -SR-. The enzyme requires no second messenger and is inhibited with high concentration of heparin, but not by inhibitors of CKI. These results indicate that this enzyme, tau-tubulin kinase is novel and distinct from TPKI, II and CKI, II.

【 授权许可】

Unknown